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nikon a1 confocal microscope  (Nikon)


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    Structured Review

    Nikon nikon a1 confocal microscope
    Nikon A1 Confocal Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 39515 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/confocal+microscope/NIS-Elements/pmc12966746-90-7-7
    Average 99 stars, based on 39515 article reviews
    nikon a1 confocal microscope - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: Regorafenib and Nifuroxazide exert enhanced suppression of hepatocellular carcinoma by inhibiting STAT3 and immune remodeling
    Article Snippet: .. Following DAPI (Beyotime Biotechnology) counterstaining at room temperature (25±2°C) for 5 min and washing with PBS, slides were mounted using an anti-fade solution, visualized and imaged using a confocal microscope (AR1; Nikon Corporation) using a ×20 objective lens. ..

    Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration
    Article Snippet: After LPS stimulation (10 μg mL −1 , 12 h, ServiceBio, GC205009 ), cells were treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h, washed twice with PBS, and incubated with JC-1 working solution (Beyotime, C2006) at 37 °C for 30 min in the dark. .. Following three PBS washes, red (aggregates) and green (monomers) fluorescence were acquired on a confocal microscope (Nikon Eclipse C2), and the red/green intensity ratio was used to assess mitochondrial membrane potential (ΔΨm). ..

    Article Title: Regorafenib and Nifuroxazide exert enhanced suppression of hepatocellular carcinoma by inhibiting STAT3 and immune remodeling
    Article Snippet: After washing with PBS, sections were incubated with the fluorescent-tagged secondary antibody (1:200; Shanghai Abways Biotechnology Co., Ltd.; cat. no. CY3101) for 30 min at room temperature. .. Following DAPI (Beyotime Biotechnology) staining at room temperature (25±2°C) for 5 min and washing with PBS, slides were mounted using an anti-fade solution, visualized and imaged using a confocal microscope (AR1; Nikon Corporation) using a ×20 objective lens. .. Apoptosis in tissues was assessed using a TUNEL assay kit (Beyotime Biotechnology).

    Article Title: A glycopeptide hydrogel confers protection and treatment in sepsis via recruitment and training of macrophages
    Article Snippet: BMDMs labeled with Cell Tracker Green CMFDA (5-Chloromethylfluorescein Diacetate) (Yeasen Biotechnology, Shanghai Co., Ltd., China) were treated with PBS, BGA, t-RADA16 or BGA@t-RADA16 for 24 h, and then were co-cultured with a fluorescent strain of E. coli for 2 h after resting for 5 days. .. The undigested E. coli was washed away with PBS, and the phagocytosis of E. coli by BMDMs was analyzed by confocal microscope (Nikon, C2). ..

    Article Title: Norepinephrine neurons in the locus coeruleus and the nucleus of the solitary tract drive different stress-related behavioral outputs in mice
    Article Snippet: .. They were then frozen and sectioned at 30 μm on a freezing microtome (HM550, Microm), mounted on microscope slides with Fluoroshield with DAPI mounting medium (F6057, Sigma-Aldrich), coverslipped, and imaged on a confocal microscope (Nikon C2). ..

    Article Title: Microfluidics-enabled proteomic profiling reveal iron-driven immune evasion by an antimicrobial-resistant pathogen.
    Article Snippet: The overnight bacteria were sub-cultured at 1:100 dilution in TB medium supplemented with ampicillin (100 mg/L) and isopropyl β-d-1thiogalactopyranoside (IPTG, 100 μM; Sigma-Aldrich) for 4 h at 33.5 ◦ C prior to the experiment. .. Next, 3 mL of YFP-E. coli in 0.01 M PBS (optical density ∼0.1; 10 8 cells/mL) was processed inside the 1.4 μm filter-height chip at 6 mL/h flow rate and observed using the confocal microscope (Nikon A1R-MP). ..

    Article Title: Aberrant dopaminergic activity during consolidation causes age-related memory generalization in Drosophila.
    Article Snippet: .. Imaging was per- formed under a confocal microscope (A1R, Nikon) with the objective positioned above the head of the fly such that the vertical lobes of the mushroom bodies were located along the z-axis. ..

    Article Title: Injectable microgels carrying engineered biomimetic nanoparticles for osteoarthritis therapy via dual-targeted senescent chondrocyte clearance and endogenous repair promotion
    Article Snippet: Cells were then washed thrice with PBS to remove unbound nanoparticles, trypsinized, and analyzed by flow cytometry (BD Accuri C6 Plus, USA). .. Furthermore, for fluorescence imaging, cells were incubated with LysoTracker (Servicebio, China) for 30 min, fixed with 4 % paraformaldehyde, counterstained with phalloidine (Beyotime, China) and DAPI (Beyotime, China), and imaged using a confocal microscope (Nikon Eclipse Ti2, Japan). .. Following cellular treatments, total RNA was isolated using TRIzol® Reagent (Thermo Fisher, USA). cDNA synthesis was performed through reverse transcription of the extracted RNA. qRT-PCR amplification was subsequently conducted using the HiScript II One Step RT-qPCR SYBR Green Kit (Vazyme, China).

    Fluorescence:

    Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration
    Article Snippet: After LPS stimulation (10 μg mL −1 , 12 h, ServiceBio, GC205009 ), cells were treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h, washed twice with PBS, and incubated with JC-1 working solution (Beyotime, C2006) at 37 °C for 30 min in the dark. .. Following three PBS washes, red (aggregates) and green (monomers) fluorescence were acquired on a confocal microscope (Nikon Eclipse C2), and the red/green intensity ratio was used to assess mitochondrial membrane potential (ΔΨm). ..

    Article Title: Injectable microgels carrying engineered biomimetic nanoparticles for osteoarthritis therapy via dual-targeted senescent chondrocyte clearance and endogenous repair promotion
    Article Snippet: Cells were then washed thrice with PBS to remove unbound nanoparticles, trypsinized, and analyzed by flow cytometry (BD Accuri C6 Plus, USA). .. Furthermore, for fluorescence imaging, cells were incubated with LysoTracker (Servicebio, China) for 30 min, fixed with 4 % paraformaldehyde, counterstained with phalloidine (Beyotime, China) and DAPI (Beyotime, China), and imaged using a confocal microscope (Nikon Eclipse Ti2, Japan). .. Following cellular treatments, total RNA was isolated using TRIzol® Reagent (Thermo Fisher, USA). cDNA synthesis was performed through reverse transcription of the extracted RNA. qRT-PCR amplification was subsequently conducted using the HiScript II One Step RT-qPCR SYBR Green Kit (Vazyme, China).

    Membrane:

    Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration
    Article Snippet: After LPS stimulation (10 μg mL −1 , 12 h, ServiceBio, GC205009 ), cells were treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h, washed twice with PBS, and incubated with JC-1 working solution (Beyotime, C2006) at 37 °C for 30 min in the dark. .. Following three PBS washes, red (aggregates) and green (monomers) fluorescence were acquired on a confocal microscope (Nikon Eclipse C2), and the red/green intensity ratio was used to assess mitochondrial membrane potential (ΔΨm). ..

    Staining:

    Article Title: Regorafenib and Nifuroxazide exert enhanced suppression of hepatocellular carcinoma by inhibiting STAT3 and immune remodeling
    Article Snippet: After washing with PBS, sections were incubated with the fluorescent-tagged secondary antibody (1:200; Shanghai Abways Biotechnology Co., Ltd.; cat. no. CY3101) for 30 min at room temperature. .. Following DAPI (Beyotime Biotechnology) staining at room temperature (25±2°C) for 5 min and washing with PBS, slides were mounted using an anti-fade solution, visualized and imaged using a confocal microscope (AR1; Nikon Corporation) using a ×20 objective lens. .. Apoptosis in tissues was assessed using a TUNEL assay kit (Beyotime Biotechnology).

    Imaging:

    Article Title: Aberrant dopaminergic activity during consolidation causes age-related memory generalization in Drosophila.
    Article Snippet: .. Imaging was per- formed under a confocal microscope (A1R, Nikon) with the objective positioned above the head of the fly such that the vertical lobes of the mushroom bodies were located along the z-axis. ..

    Article Title: Injectable microgels carrying engineered biomimetic nanoparticles for osteoarthritis therapy via dual-targeted senescent chondrocyte clearance and endogenous repair promotion
    Article Snippet: Cells were then washed thrice with PBS to remove unbound nanoparticles, trypsinized, and analyzed by flow cytometry (BD Accuri C6 Plus, USA). .. Furthermore, for fluorescence imaging, cells were incubated with LysoTracker (Servicebio, China) for 30 min, fixed with 4 % paraformaldehyde, counterstained with phalloidine (Beyotime, China) and DAPI (Beyotime, China), and imaged using a confocal microscope (Nikon Eclipse Ti2, Japan). .. Following cellular treatments, total RNA was isolated using TRIzol® Reagent (Thermo Fisher, USA). cDNA synthesis was performed through reverse transcription of the extracted RNA. qRT-PCR amplification was subsequently conducted using the HiScript II One Step RT-qPCR SYBR Green Kit (Vazyme, China).

    Incubation:

    Article Title: Injectable microgels carrying engineered biomimetic nanoparticles for osteoarthritis therapy via dual-targeted senescent chondrocyte clearance and endogenous repair promotion
    Article Snippet: Cells were then washed thrice with PBS to remove unbound nanoparticles, trypsinized, and analyzed by flow cytometry (BD Accuri C6 Plus, USA). .. Furthermore, for fluorescence imaging, cells were incubated with LysoTracker (Servicebio, China) for 30 min, fixed with 4 % paraformaldehyde, counterstained with phalloidine (Beyotime, China) and DAPI (Beyotime, China), and imaged using a confocal microscope (Nikon Eclipse Ti2, Japan). .. Following cellular treatments, total RNA was isolated using TRIzol® Reagent (Thermo Fisher, USA). cDNA synthesis was performed through reverse transcription of the extracted RNA. qRT-PCR amplification was subsequently conducted using the HiScript II One Step RT-qPCR SYBR Green Kit (Vazyme, China).



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